Methylene Blue

Chemical Name: 3,7-Bis(dimethylamino)-phenothiazin-5-ium chloride

CAS Number: 61-73-4

Molecular Formula: C16H18ClN3S

Appearance: Dark green crystalline powder or dark blue solution

Solubility: Soluble in water and alcohol; forms a blue solution.

Product Overview:

Methylene Blue is a synthetic dye and medication with various applications in biological research, medicine, and industrial fields. As a versatile stain, it is widely used in microbiology and histology for staining cells, tissues, and microorganisms. Methylene Blue also has therapeutic uses, particularly in treating methemoglobinemia, a condition where hemoglobin is unable to carry oxygen effectively.

In addition to its biological and medical uses, Methylene Blue has been utilized in the textile and chemical industries as a colorant.

Key Features:

• Wide Range of Applications: Used in both laboratory settings for staining and in clinical settings for its therapeutic properties.
• Versatile Staining Agent: Effective in visualizing various biological specimens, including bacteria, cells, and tissues, especially in microscopy.
• Medical Use: Known for its role in treating conditions like methemoglobinemia, urinary tract infections, and as an antidote to certain poisons.
• Stability: Methylene Blue is stable under proper storage conditions and can be stored for long periods without significant degradation.

Applications:

• Biological Staining: Methylene Blue is one of the most common stains in microbiology and histology, used for staining living and dead cells. It’s also used for Gram staining, helping to differentiate bacterial species.
• Medical Uses: Methylene Blue is utilized as a medication to treat methemoglobinemia, a condition in which oxygen is not effectively released from hemoglobin to body tissues. It’s also used as a treatment for urinary tract infections, and as a dye in diagnostic procedures.
• Textile Industry: Employed to dye fabrics and materials, providing a stable, blue color.
• Redox Indicator: Used as a redox indicator in chemical experiments due to its ability to change color depending on its oxidation state.

Storage and Handling:

• Storage Conditions: Keep in a tightly sealed container in a cool, dry, and well-ventilated area, away from direct sunlight.
• Safety Precautions: Use personal protective equipment (PPE), including gloves, goggles, and protective clothing, when handling Methylene Blue. Ensure proper ventilation in the work area.
• Handling: Avoid inhalation of the dust and direct contact with eyes and skin. In case of contact, rinse thoroughly with water.